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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

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Related Experiment Video

Updated: Jun 14, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Conserved nucleosome positioning defines replication origins.

Matthew L Eaton¹, Kyriaki Galani, Sukhyun Kang

¹Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.

Genes & Development

|March 31, 2010

Summary

This summary is machine-generated.

The origin recognition complex (ORC) binds DNA replication origins. Nucleosomes, which package DNA, precisely position around these origins, influencing where DNA replication begins.

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

The origin recognition complex (ORC) is crucial for initiating DNA replication.
In Saccharomyces cerevisiae, ORC binds to autonomously replicating sequence (ARS) consensus sequences (ACS).
However, not all ACS sites are bound by ORC, indicating other factors influence origin selection.

Purpose of the Study:

To investigate the role of chromosomal features, specifically nucleosome positioning, in ORC binding and origin selection.
To understand how nucleosomes influence the precise location and function of DNA replication origins.

Main Methods:

Utilized high-throughput sequencing to map ORC binding sites across the genome.
Employed sequencing to determine nucleosome positioning relative to ORC binding sites.
Analyzed the sufficiency of origin sequences for nucleosome-free regions and the role of ORC in nucleosome positioning.

Main Results:

Yeast replication origins exhibit a distinct asymmetric pattern of positioned nucleosomes flanking the ACS.
Origin sequences alone can establish a nucleosome-free region.
ORC is essential for the accurate positioning of nucleosomes adjacent to the replication origin.

Conclusions:

Local nucleosome positioning is a critical determinant for selecting and regulating DNA replication origins.
The interplay between ORC and nucleosomes fine-tunes the initiation of DNA replication.
This study reveals a novel epigenetic mechanism controlling genome replication.