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Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
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Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

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New automated hepatitis C virus (HCV) core antigen assay as an alternative to real-time PCR for HCV RNA

Yongjung Park1, Jong-Han Lee, Beom Seok Kim

  • 1Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsan-no, Seodaemun-gu, Seoul 120-752, South Korea.

Journal of Clinical Microbiology
|March 31, 2010
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...