Related Experiment Video
Updated: Jun 14, 2026

08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Modelling and simulation revealing mechanisms likely responsible for achieving the nitrite pathway through aeration
A Guisasola1, M Marcelino, R Lemaire
1Departament d'Enginyeria Química, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain. albert.guisasola@uab.cat
Summary
Nitrogen removal via nitrite offers cost savings. Competition for nitrite, not kinetics, drives nitrite-oxidizing bacteria washout, aiding efficient nitrogen removal.
Area of Science:
- Environmental microbiology
- Wastewater treatment engineering
- Biochemical process modeling
Background:
- Nitrogen removal via nitrite is increasingly favored over nitrate for reduced aeration and chemical oxygen demand (COD) costs.
- Established control strategies, including aerobic phase length and low dissolved oxygen (DO) setpoints, facilitate the nitrite pathway in sludge-retention systems.
Purpose of the Study:
- To investigate the role of microbial competition versus kinetic selection in nitrite-oxidizing bacteria (NOB) washout.
- To evaluate the impact of simultaneous nitrification and denitrification (SND) on nitrite pathway efficiency and COD utilization.
Main Methods:
- Utilizing mathematical modeling and simulation to analyze microbial dynamics.
- Examining the competition between nitrite-reducing and nitrite-oxidizing bacteria for nitrite.
Main Results:
- Competition for nitrite, not kinetic selection, is the primary factor causing NOB washout.
- Simultaneous nitrification and denitrification under aerobic conditions significantly aids nitrite pathway achievement.
- Enhanced COD utilization is observed with the occurrence of SND.
Conclusions:
- Understanding nitrite competition is crucial for optimizing nitrogen removal processes.
- SND presents a promising strategy for improving the efficiency of biological nitrogen removal.
- The nitrite pathway offers substantial economic and environmental benefits in wastewater treatment.
Related Concept Videos
Microbes and the Nitrogen Cycle
The nitrogen cycle is a complex biogeochemical process critical to maintaining the balance of nitrogenous compounds in ecosystems. This cycle involves multiple microbial-mediated transformations through which nitrogen changes oxidation states, supporting essential ecological functions and contributing to plant and microbial growth.Nitrogen Fixation and AmmonificationNitrogen fixation initiates the cycle by converting inert atmospheric nitrogen (N₂) into bioavailable ammonia (NH₃), a process...
Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Inorganic Nitrogen Assimilation
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...
Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Metabolism of Chemolithotrophs
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...

