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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 14, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Recent advances in enzyme assays using fluoremetry].

Yanlong Xing¹, Xiangzhao Mao, Shu Wang

¹Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Science, Qingdao 266101, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|April 1, 2010

Summary

This summary is machine-generated.

Fluorescent probes offer sensitive and dynamic monitoring for enzyme assays, crucial for bio-engineering, disease diagnosis, and drug discovery. This review covers recent advances and applications of these powerful tools.

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Last Updated: Jun 14, 2026

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Area of Science:

Biochemistry and Molecular Biology
Analytical Chemistry

Context:

Enzyme assays are critical tools in various scientific and medical fields.
Fluorescence-based detection methods are gaining prominence due to their high sensitivity and dynamic monitoring capabilities.

Purpose:

To review recent advancements and applications of fluorescent probes in enzyme assays.
To classify different fluorescence detection methods based on substrates and mechanisms.
To provide an overview of available tools and strategies for enzyme assays using fluorescent probes.

Summary:

This review categorizes enzyme assay methods using fluorescent probes into direct and indirect detection.
It highlights the advantages of fluorometry, including sensitivity and real-time monitoring.
The discussion encompasses current research trends and future prospects in the field.

Impact:

Facilitates the development of novel diagnostic tools for diseases.
Supports advancements in drug discovery and development pipelines.
Provides researchers with a comprehensive understanding of fluorescent probe applications in enzyme analysis.