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[Genetic diversity of Changium smyrnioides based on SRAP]
Changlin Wang1, Qiaosheng Guo, Yumei Wu
1Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Objective:
To study the genetic diversity of Changium smyrnioides and give a reference for utilization of the germplasm.
Method:
Ten different populations of Ch. smyrnioides were analyzed by the approach of sequence-related amplified polymorphism (SRAP). Genetic similarity coefficient was calculated, and systematic relationships were constructed based on the UPGMA method.
Result:
Seventeen primer pairs were selected from 160. A total of 363 bands were scored, 314 bands of them were polymorphic and the average was 18.47 polymorphic bands per primer pair, which were up to 86.50% polymorphic ratio. The results indicated that there was abundant genetic diversity among the tested materials. Genetic similarity coefficient was ranged from 0.4959 to 0.8182. Cluster analysis showed that ten different populations of Ch. smyrnioides could be distinguished into two groups.
Conclusion:
High level genetic diversity was in different populations of Ch. smyrnioides, and genetic relationship was correlative to geographic position.
