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Chemical constituents of Periploca forrestii and their cytotoxicity activity
Jin Wang1, Cuifang Wang, Jinxiong Chen
1The State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sinences, Kunming 650204, China.
Objective:
To investigate the chemical constituents of the roots of Periploca forrestii and evaluate their cytotoxicity activities.
Method:
Silica gel column chromatography was employed for the isolation and purification of chemical constituents. The structures were identified on the basis of spectral data and the cytotoxicities of compounds 2-4 were investigated by several tumors cell lines including blood tumor (HL-60, CCRT-CEM), prostate tumor (PC-3, DU-145) and Melanoma (UACC-62).
Result:
Four compounds were isolated and identified as follows, lupeol-20(29)-en-3-nonadecanoate (1), peroiforoside I (2), 3beta,5beta,14beta-3OH-8beta-H-car-20(22)-enolide (3), perplocin (4).
Conclusion:
Compound 1 is a new lupane triterpene fatty acid ester. Compounds 2-4 showed notable cytotoxicity against all tumor lines.