Related Experiment Video
Updated: Jun 14, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
Published on: June 7, 2020
[Isoflavones from vines of Pueraria lobata]
Dewu Zhang1, Yan Ren, Shengjun Dai
1School of Pharmaceutical Science, Yantai University, Yantai 264005, China.
Objective:
To investigate the isoflavones from the vines of Pueraria lobata.
Method:
The compounds were isolated by column chromatography over silica gel and RP-C18, and purified by Sephadex LH-20 column chromatography and preparative TLC. The structures were elucidated on the basis of physico-chemical properties and spectral data.
Result:
Twelve compounds were isolated and identified as: 3'-methoxydaidzein (1), formononetin (2), genistein (3), daidzein (4), daidzin (5), genistin (6), ononin (7), 5-hydroxyl ononin (8), calycosin (9), 6"-O-acetyl genistein (10), 6"-O-acetyl daidzin (11), puerarin (12).
Conclusion:
For the first time, compounds 9-11 were isolated from the genus Pueraria plant, and compounds 1, 3, 6-8 were obtained from the vines of this plant.
Related Concept Videos
Cancer Prevention
Some...
Plant Hormones
Plant Hormones

