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Hypolipidemic effect of Pleurotus eryngii extract in fat-loaded mice
Tomohiro Mizutani1, Satoshi Inatomi, Akihiro Inazu
1Mushroom Research Laboratory, Hokuto Corporation, Nagano, Japan.
Abstract:
Pleurotus eryngii water extract (PEE), which showed the most significant inhibitory activity against pancreatic lipase in vitro among eight edible mushrooms, was investigated to determine the mechanism of its anti-lipase activity in vitro and its hypolipidemic effect in fat-loaded mice. The inhibitory effects of mushroom extracts on pancreatic lipase activity were examined using 4-methylumbelliferyl oleate (4-MUO) or trioleoylglycerol emulsified with lecithin, gum arabic or Triton X-100 as a substrate. For in vivo experiments, blood samples were taken after oral administration of corn oil and [(3)H]trioleoylglycerol with or without PEE to food-deprived mice. PEE inhibited hydrolysis of 4-MUO and trioleoylglycerol emulsified with lecithin or Triton X-100, but not that of trioleoylglycerol emulsified with gum arabic. PEE suppressed the elevations of plasma and chylomicron triacylglycerol levels after oral administration of corn oil, but had no effect on lipoprotein lipase activity. [(3)H]Trioleoylglycerol absorption was also decreased by administration of PEE. The results of in vitro studies suggest that PEE may prevent interactions between lipid emulsions and pancreatic lipase. The hypolipidemic effect of PEE in fat-loaded mice may be due to low absorption of fat caused by the inhibition of pancreatic lipase.
Insights
Pleurotus eryngii water extract (PEE) inhibits pancreatic lipase, reducing fat absorption and lowering blood lipids in mice. This mushroom extract shows potential for managing hyperlipidemia by affecting fat digestion.
Area of Science:
- Pharmacology
- Biochemistry
- Nutritional Science
Background:
- Pancreatic lipase is a key enzyme in dietary fat digestion.
- Obesity and hyperlipidemia are significant global health concerns.
- Natural compounds are being explored for their potential therapeutic effects.
Purpose of the Study:
- To investigate the anti-lipase mechanism of Pleurotus eryngii water extract (PEE).
- To evaluate the hypolipidemic effects of PEE in vivo.
- To determine if PEE can reduce fat absorption.
Main Methods:
- In vitro assays using various substrates to measure pancreatic lipase inhibition.
- In vivo studies in fat-loaded mice administering corn oil and radiolabeled trioleoylglycerol with or without PEE.
- Analysis of plasma triacylglycerol levels and chylomicron formation.
- Assessment of lipoprotein lipase activity.
Main Results:
- PEE demonstrated significant in vitro inhibition of pancreatic lipase, particularly with lecithin or Triton X-100 emulsified substrates.
- PEE administration in mice reduced plasma and chylomicron triacylglycerol levels after a fat load.
- PEE decreased the absorption of orally administered fat.
- No effect of PEE was observed on lipoprotein lipase activity.
Conclusions:
- PEE inhibits pancreatic lipase by interfering with lipid emulsion and enzyme interaction.
- The hypolipidemic effect of PEE in vivo is attributed to reduced fat absorption due to pancreatic lipase inhibition.
- Pleurotus eryngii extract shows promise as a natural agent for managing hyperlipidemia.
