Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Degenerative Disc Disease I: Introduction01:27

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...
Degenerative Disc Disease ll: Pathophysiology01:23

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...
Herniated Intervertebral Disc l: Introduction01:29

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphometric analysis reveals that the chick cranial neural tube expands as an active shell.

bioRxiv : the preprint server for biology·2026
Same author

Mechanical cues from muscle contraction regulate TGFβ signaling and epitenon formation during embryonic tendon development.

bioRxiv : the preprint server for biology·2026
Same author

An endodermal subpopulation generates neural and mesodermal fates in the posterior chick embryo.

bioRxiv : the preprint server for biology·2026
Same author

Vertebral Body Kinematics Measured From T1-Weighted Magnetic Resonance Imaging With Optimized Rigid Registration.

Journal of biomechanical engineering·2026
Same author

Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon Repair.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Advances in mechanical assessments of in vivo human lumbar spine tissues with noninvasive imaging techniques.

npj biomedical innovations·2026
Same journal

Comparing those Most Satisfied versus Least Satisfied Following Surgery for Cervical Spondylotic Myelopathy: Are there Differences in Baseline Characteristics?

Spine·2026
Same journal

Pseudoarthrosis After Posterior Spinal Fusion in Adolescent Idiopathic Scoliosis: A Multicenter Analysis of Revision Strategies and Outcomes.

Spine·2026
Same journal

To the Editor "Low-Density Lipoprotein Cholesterol and Statin Usage Are Associated With Rates of Pseudarthrosis Following Single-Level Posterior Lumbar Interbody Fusion" by Lavu et al.

Spine·2026
Same journal

Sarcopenia Increases Adjacent Segment Degeneration Risk within 3 Years of Anterior Cervical Discectomy and Fusion.

Spine·2026
Same journal

Two-Year Cervical Alignment Trajectories and Associated Radiographic Factors after Posterior Spinal Fusion for Lenke Type 1 Adolescent Idiopathic Scoliosis.

Spine·2026
Same journal

Association of C7 Laminoplasty and Decompression Construct Length With Postoperative Axial Symptoms After Cervical Expansive Unilateral Open-door Laminoplasty.

Spine·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

Engineered disc-like angle-ply structures for intervertebral disc replacement.

Nandan L Nerurkar1, Sounok Sen, Alice H Huang

  • 1Department of Orthopaedic Surgery, McKay Orthopaedic Research Laboratory, University of Pennsylvania, Philadelphia, PA, USA.

Spine
|April 1, 2010
PubMed
Summary
This summary is machine-generated.

This study engineered a novel mesenchymal stem cell (MSC)-based disc replacement using a unique angle-ply structure (DAPS). The DAPS successfully replicated the intervertebral disc's complex architecture and mechanical properties, offering a promising foundation for future disc repair.

More Related Videos

Multilevel Oblique Lumbar Interbody Fusion in Degenerative Lumbar Disc Disease with Instability
11:30

Multilevel Oblique Lumbar Interbody Fusion in Degenerative Lumbar Disc Disease with Instability

Published on: July 25, 2025

Lateral-PLIF for Lumbar Spinal Arthrodesis: A Detailed Step-By-Step Surgical Technique
08:36

Lateral-PLIF for Lumbar Spinal Arthrodesis: A Detailed Step-By-Step Surgical Technique

Published on: January 23, 2026

Related Experiment Videos

Last Updated: Jun 14, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

Multilevel Oblique Lumbar Interbody Fusion in Degenerative Lumbar Disc Disease with Instability
11:30

Multilevel Oblique Lumbar Interbody Fusion in Degenerative Lumbar Disc Disease with Instability

Published on: July 25, 2025

Lateral-PLIF for Lumbar Spinal Arthrodesis: A Detailed Step-By-Step Surgical Technique
08:36

Lateral-PLIF for Lumbar Spinal Arthrodesis: A Detailed Step-By-Step Surgical Technique

Published on: January 23, 2026

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Engineering a functional intervertebral disc (IVD) replacement requires replicating its complex mechanical function and anatomical form.
  • Previous attempts have not replicated the native disc's multiscale hierarchical architecture or fully assessed neo-tissue mechanical function.
  • Degenerative disc disease necessitates innovative solutions for effective IVD replacement.

Purpose of the Study:

  • To engineer a disc-like angle-ply structure (DAPS) that mimics the native intervertebral disc's multiscale architecture.
  • To develop a construction algorithm coupling electrospun nanofibrous scaffolds with biocompatible hydrogels for mesenchymal stem cell (MSC)-based disc engineering.
  • To create a composite disc analogue for potential disc replacement applications.

Main Methods:

  • Developed a novel construction algorithm utilizing agarose for the nucleus pulposus (NP) and oriented electrospun nanofibrous scaffolds for the anulus fibrosus (AF).
  • Seeded bovine MSCs into both NP and AF regions of the engineered constructs.
  • Evaluated construct maturation through biochemical, histologic, and mechanical assessments during in vitro culture.

Main Results:

  • Mechanical testing (compression, torsion) demonstrated equilibrium and time-dependent behaviors qualitatively similar to native IVD tissue.
  • Seeded cells adopted distinct morphologies in AF and NP regions, mirroring native tissue.
  • Cells in the AF region organized matrix deposition in an angle-ply configuration, replicating native architecture.
  • Constructs exhibited functional development during long-term in vitro culture.

Conclusions:

  • The developed DAPS offers a new approach for intervertebral disc tissue engineering.
  • The method successfully replicates the multiscale form and function of the native disc.
  • This provides a foundation for creating a biologically relevant, anatomically accurate, and hierarchically organized composite disc analogue for eventual disc replacement.