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Related Experiment Video

Updated: Jun 14, 2026

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

The 'when and whereabouts' of injected pathogen effectors.

Cristina D Rodrigues¹, Jost Enninga

¹Groupe Dynamique des interactions hôte-pathogène, Institut Pasteur, Paris, France.

|April 1, 2010

Summary

A novel split green fluorescent protein (GFP) method allows researchers to track bacterial effector proteins within single living host cells. This breakthrough enables detailed observation of effector dynamics during host-pathogen interactions.

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Area of Science:

Microbiology
Cell Biology
Molecular Biology

Background:

Bacterial effector proteins are crucial virulence factors that manipulate host cell functions.
Understanding the spatiotemporal dynamics of these effectors is essential for deciphering host-pathogen interactions.
Previous methods lacked the resolution to track individual effector molecules in real-time within living cells.

Discussion:

The split GFP system provides a sensitive and specific tool for visualizing bacterial effector translocation.
This technique allows for the real-time monitoring of effector movement and localization within host cells.
The method overcomes limitations of traditional techniques, offering unprecedented insights into effector trafficking.

Key Insights:

Developed a split GFP-based assay for tracking bacterial effector proteins in single living host cells.
Demonstrated the ability to visualize the spatiotemporal dynamics of effector injection and intracellular movement.
This advancement facilitates the study of effector function and host cell manipulation at the single-cell level.

Outlook:

Future applications may involve studying a wider range of bacterial pathogens and their effectors.
This method can be adapted to investigate other protein trafficking events in host-pathogen systems.
Potential for drug discovery by identifying host targets or effector functions critical for pathogenesis.