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Published on: March 9, 2015
X-linked insertion/deletion polymorphisms: forensic applications of a 33-markers panel
Natalle S C Freitas1, Rafael L Resque, Elzemar M Ribeiro-Rodrigues
1Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Cidade Universitária Prof. José da Silva Netto. Av. Augusto Corrêa, 01, Belém, PA, Brazil.
This study introduces a new method for analyzing X-chromosome insertion/deletion (INDEL) polymorphisms in forensic science. The developed X-INDEL panel is effective for genotyping mixed populations, yielding reliable biostatistical parameters for forensic applications.
Area of Science:
- Forensic genetics
- Population genetics
- Anthropology
Background:
- Insertion/deletion (INDEL) polymorphisms offer advantages for forensic and anthropological studies.
- X-chromosome linked INDELs (X-INDELs) present unique genotyping opportunities.
- Current methods require efficient multiplexing for broad applicability.
Purpose of the Study:
- To develop a laboratory methodology for analyzing 33 X-INDEL markers in a single PCR multiplex reaction.
- To evaluate the utility of this X-INDEL panel for forensic applications in a Brazilian Amazonian population.
- To assess the compatibility of biostatistical parameters derived from X-INDELs with existing forensic standards.
Main Methods:
- Development of a multiplex PCR assay for 33 X-INDEL markers.
- Capillary electrophoresis for fragment analysis and genotyping.
- Genotyping of 351 individuals from a mixed Brazilian Amazonian population.
Main Results:
- Successful development and implementation of the 33 X-INDEL multiplex assay.
- Efficient genotyping of individuals from a mixed population.
- Biostatistical parameters obtained were comparable to those from other populations using X-STR panels.
Conclusions:
- The developed X-INDEL panel provides a robust tool for forensic genetics.
- This methodology is suitable for analyzing mixed populations, like those in the Brazilian Amazon.
- X-INDEL markers are valuable for forensic investigations and anthropological research.
