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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Screening sweetcorn for enhanced zeaxanthin concentration.

Kent J Fanning1, Ian Martin, Lung Wong

  • 1Innovative Food Technologies, Primary Industries and Fisheries, Department of Employment, Economic Development and Innovation, 19 Hercules Street, Hamilton, Queensland, Australia, 4007. kent.fanning@deedi.qld.gov.au

Journal of the Science of Food and Agriculture
|April 1, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to screen for high zeaxanthin sweetcorn. This method identified breeding lines with significantly higher zeaxanthin levels than commercial varieties, aiding in developing healthier crops.

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Area of Science:

  • Agricultural Science
  • Food Science
  • Biochemistry

Background:

  • Development of new fruit and vegetable varieties aims to enhance consumer health benefits through increased carotenoid levels.
  • A specific focus is on developing high zeaxanthin sweetcorn varieties.

Purpose of the Study:

  • To develop and apply an analytical screening method for high zeaxanthin sweetcorn.
  • To assess carotenoid levels in a breeding population and commercial varieties.

Main Methods:

  • Chromameter measurement of hue angle.
  • Optimized extraction for High-Performance Liquid Chromatography (HPLC).
  • Application to 385 breeding lines and six commercial sweetcorn varieties.

Main Results:

  • Saponification did not impact carotenoid extraction efficiency.
  • Breeding lines exhibited a wide range of carotenoid levels, with the highest zeaxanthin at 11.9 mg kg(-1) FW, six times greater than commercial varieties.
  • A regression model (hue angle = 76.16 + 4.50 x exp(-0.24 x zeaxanthin) + 11.73 x exp(-0.24 x zeaxanthin), r(2) = 0.59) was established.
  • Top 6% of lines for zeaxanthin and total carotenoids had hue angles ≤84.1 degrees.

Conclusions:

  • A hue angle cut-off of 85 degrees for liquid extraction can significantly improve screening efficiency for high zeaxanthin germplasm.
  • There is substantial potential to increase zeaxanthin concentration by selecting lines that preferentially channel carotenoid synthesis towards zeaxanthin.