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Updated: Jun 14, 2026

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
PEGylated Chromatography: Efficient Bioseparation on Silica Monoliths Grafted with Smart Biocompatible Polymers
Irene Tan1, Zoya Zarafshani, Jean-François Lutz
1Colloid Chemistry Department, Max-Planck Institute of Colloids and Interfaces, Scientific Campus Golm, 14476 Potsdam, Germany.
Abstract:
Novel oligo(ethylene glycol)-based thermoresponsive stationary phases have been studied for the separation of bioanalytes. Well-defined copolymers of (2-methoxyethoxy)ethyl methacrylate and oligo(ethylene glycol) methacrylate were synthesized by atom-transfer radical polymerization in the presence of an N-succinimidyl-functionalized initiator. The reactive chain ends of these copolymers were then reacted with amino-functionalized silica monoliths. The formed composites were studied as chromatography materials. For instance, it was demonstrated that thermoresponsive oligo(ethylene glycol)-based stationary phases allow rapid, efficient separation of steroid and protein mixtures in pure water under isocratic high-performance liquid chromatographic elution.
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