Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

Polytene Chromosomes

Polytene Chromosomes

Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...

The DNA Helix

The DNA Helix

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Emergency carotid artery stenting during endovascular treatment of tandem lesions in acute ischemic stroke: a single-center retrospective study.

CVIR endovascular·2026

Same author

Operational classification of cutaneous squamous cell carcinomas based on unsupervised clustering of real cases by experts.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024

Same author

Exploring the most visible websites on cutaneous T-cell lymphoma-revealing limited quality of patient health information on the internet.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022

Same author

Impact of the Covid-19 pandemic on melanoma and non-melanoma skin cancer inpatient treatment in Germany - a nationwide analysis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022

Same author

Vascular 3D Printing with a Novel Biological Tissue Mimicking Resin for Patient-Specific Procedure Simulations in Interventional Radiology: a Feasibility Study.

Journal of digital imaging·2022

Same author

PD-1 - blockade in advanced cutaneous squamous cell carcinoma - fresh breeze in a deadly lull.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021

Same journal

Removal of Codispersible Residual Impurities from CuInS<sub>2</sub>/ZnS Quantum Dots for Window-Replaceable Luminescent Solar Concentrators.

ACS applied materials & interfaces·2026

Same journal

Durable Core-Shell Scatterer Coating with Heat Storage for Radiative Cooling.

ACS applied materials & interfaces·2026

Same journal

Calix[6]arene-Based Interlocked Inverse Vulcanizate Enabling Network-Interface Cooperative Reinforcement in Natural Rubber/Carbon Black Composites.

ACS applied materials & interfaces·2026

Same journal

Resolving Thermal Accumulation and Rigid-Soft Interface Mismatch in Stretchable Electronics with Cubic Boron Nitride Composite Islands.

ACS applied materials & interfaces·2026

Same journal

Enhancing Conversion Reversibility and Initial Coulombic Efficiency of SnO<sub>2</sub> Anodes via NiO/Ni-Carbon Interfacial Design.

ACS applied materials & interfaces·2026

Same journal

Multidimensional Interface Structure Design for High-Efficiency Optically Controlled Semiconductor Devices: A Case Study on Memristive Synapses.

ACS applied materials & interfaces·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Three-dimensional surface patterning by DNA-modifying enzymes.

R Kaufmann¹, D Peled, R Naaman

¹Department of Chemical Physics and Chemical Research Support, Weizmann Institute of Science, Rehovot 76100, Israel.

ACS Applied Materials & Interfaces

|April 2, 2010

Summary

This summary is machine-generated.

Researchers demonstrate self-assembled patterned multilayers using DNA monolayers and enzymes. This method enables precise fabrication of complex structures through controlled DNA modification and nucleotide incorporation.

More Related Videos

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Related Experiment Videos

Last Updated: Jun 14, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Area of Science:

Biomolecular engineering
Nanotechnology
Surface chemistry

Background:

DNA monolayers offer a versatile platform for surface functionalization.
Enzyme-catalyzed reactions provide high specificity for molecular assembly.
Fabricating ordered multilayered structures is crucial for advanced materials.

Purpose of the Study:

To demonstrate the fabrication of self-assembled patterned multilayers using DNA monolayers and enzymes.
To showcase the quantitative and selective nature of enzyme-mediated DNA modification.
To explore the potential of this approach for supramolecular structure fabrication.

Main Methods:

Formation of DNA monolayers on silicon surfaces.
Quantitative DNA cleavage using restriction enzymes.
Covalent incorporation of fluorescently labeled nucleotides to cleaved DNA.
Characterization of nucleotide addition selectivity and surface adsorption.

Main Results:

DNA monolayers were successfully cleaved by restriction enzymes.
Fluorescently labeled nucleotides were selectively incorporated based on DNA sequence.
No nonspecific adsorption of nucleotides to the surface was observed.
The enzyme-mediated dual action was quantitative, enabling multilayer fabrication.

Conclusions:

DNA monolayers and modifying enzymes provide a robust method for self-assembled multilayer fabrication.
Enzyme-directed nucleotide incorporation ensures high precision and specificity.
This technique expands the toolkit for creating complex supramolecular structures.