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Updated: Aug 6, 2026
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Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
Published on: October 27, 2009
Antibody-detectable changes in fibrinogen adsorption affecting platelet activation on polymer surfaces
E Shiba1, J N Lindon, L Kushner
1Beth Israel Hospital, Boston 02215.
The American Journal of Physiology
|May 1, 1991
Summary
Platelet interaction with artificial surfaces depends on fibrinogen organization. Specific fibrinogen regions and platelet receptors (GpIIb/IIIa) are crucial for this interaction, influencing platelet retention on methacrylate polymers.
Area of Science:
- Biomaterials Science
- Hematology
- Surface Chemistry
Background:
- Platelet reactivity with artificial surfaces correlates with adsorbed fibrinogen concentration.
- The organization of adsorbed fibrinogen influences platelet interactions.
Purpose of the Study:
- To investigate how fibrinogen organization on hydrophobic surfaces affects platelet binding.
- To determine the role of specific fibrinogen epitopes and platelet receptors in this process.
Main Methods:
- Studied antibody binding to adsorbed fibrinogen on polyalkyl methacrylate polymers.
- Compared antibody binding with platelet retention in methacrylate bead columns.
- Utilized polyclonal and monoclonal antibodies against fibrinogen and its epitopes, and against the platelet receptor GpIIb/IIIa.
Main Results:
- Diluted plasma pretreatment increased platelet retention.
- Polyclonal anti-fibrinogen antibodies and antibodies against a specific gamma-peptide eliminated retention.
- Monoclonal antibodies against other COOH-terminal epitopes reduced retention.
- An antibody against the GpIIb/IIIa receptor completely inhibited platelet retention.
Conclusions:
- Methacrylate polymers alter adsorbed fibrinogen, impacting platelet interaction.
- Platelet retention involves the COOH-terminal end of fibrinogen's gamma-chain interacting with platelet GpIIb/IIIa receptors.
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