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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

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Published on: August 16, 2016

A novel electrolyte-responsive membrane with tunable permeation selectivity for protein purification.

Yong-Hong Zhao1, Kin-Ho Wee, Renbi Bai

  • 1Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore.

ACS Applied Materials & Interfaces
|April 2, 2010
PubMed
Summary

This study introduces a novel electrolyte-responsive membrane, RC-g-PSBMA, capable of separating proteins from nanoparticles. The membrane

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Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biotechnology

Background:

  • Regenerated cellulose (RC) membranes are widely used in separation processes.
  • Developing smart membranes with tunable properties is crucial for advanced applications.
  • Zwitterionic polymers offer unique responsive characteristics for membrane technology.

Purpose of the Study:

  • To synthesize and characterize a novel electrolyte-responsive membrane, RC-g-PSBMA.
  • To evaluate the membrane's performance in separating proteins and nanoparticles.
  • To demonstrate the potential of this membrane for selective purification applications.

Main Methods:

  • Surface-initiated atom transfer radical polymerization (ATRP) of sulfobetaine methacrylate (SBMA) onto RC membranes.
  • Preparation of RC-g-PSBMA with varying degrees of polymerization.
  • Permeation experiments using sodium chloride (NaCl) solutions of different concentrations.
  • Separation studies involving bovine serum albumin (BSA) and polystyrene nanoparticles (NPs) of various sizes.

Main Results:

  • RC-g-PSBMA exhibited electrolyte-responsive permeability dependent on NaCl concentration.
  • The membrane showed low rejection rates for BSA, allowing protein permeation.
  • Rejection rates for NPs significantly varied with NaCl concentration, enabling size-dependent separation.
  • Successful separation of BSA from NPs was achieved by adjusting NaCl concentration.

Conclusions:

  • The developed RC-g-PSBMA membrane demonstrates tunable separation capabilities based on electrolyte concentration.
  • This smart membrane offers a promising platform for selective protein purification and other separation challenges.
  • The ability to control separation by simply altering salt concentration opens new avenues in membrane technology.