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11 beta-Hydroxysteroid dehydrogenase mRNA expression in rat kidney
J L Yau1, A D Van Haarst, M P Moisan
1Department of Medicine, University of Edinburgh, Western General Hospital, United Kingdom.
The American Journal of Physiology
|May 1, 1991
Summary
11 beta-Hydroxysteroid dehydrogenase (11 beta-OHSD) in rat kidneys converts corticosterone to inactive 11-dehydrocorticosterone, protecting mineralocorticoid receptors. This enzyme
Area of Science:
- Renal physiology
- Steroid hormone metabolism
- Molecular biology
Background:
- 11 beta-Hydroxysteroid dehydrogenase (11 beta-OHSD) is crucial for protecting renal mineralocorticoid receptors from glucocorticoids.
- While 11 beta-OHSD activity and mineralocorticoid receptors are present in distal tubules, their precise colocalization is unclear.
- Multiple kidney isoforms of 11 beta-OHSD exist, but only one mRNA species has been identified.
Purpose of the Study:
- To investigate the localization of 11 beta-Hydroxysteroid dehydrogenase mRNA in rat renal tubular epithelia.
- To determine if 11 beta-OHSD mRNA colocalizes with mineralocorticoid receptors in distal tubular cells.
Main Methods:
- In situ hybridization was employed to detect 11 beta-OHSD mRNA expression.
- Analysis focused on the distribution of mRNA within different segments of the rat kidney tubules.
Main Results:
- 11 beta-Hydroxysteroid dehydrogenase mRNA was found to be highly expressed in all renal tubular epithelia.
- The distribution pattern suggests a colocalization of 11 beta-OHSD mRNA with mineralocorticoid receptors in distal tubular cells.
Conclusions:
- 11 beta-Hydroxysteroid dehydrogenase mRNA is widely distributed throughout rat renal tubular epithelia.
- This widespread expression supports the hypothesis that 11 beta-OHSD is colocalized with mineralocorticoid receptors, playing a key role in renal mineralocorticoid balance.