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Whole-genome characterization and genotyping of global WU polyomavirus strains
Seweryn Bialasiewicz1, Rebecca Rockett, David W Whiley
1Sir Albert Sakzewski Virus Research Centre, Building C28, Back Rd., Herston, Queensland 4029, Australia. seweryn@uq.edu.au
Abstract:
Exploration of the genetic diversity of WU polyomavirus (WUV) has been limited in terms of the specimen numbers and particularly the sizes of the genomic fragments analyzed. Using whole-genome sequencing of 48 WUV strains collected in four continents over a 5-year period and 16 publicly available whole-genome sequences, we identified three main WUV clades and five subtypes, provisionally termed Ia, Ib, Ic, II, IIIa, and IIIb. Overall nucleotide variation was low (0 to 1.2%). The discriminatory power of the previous VP2 fragment typing method was found to be limited, and a new, larger genotyping region within the VP2/1 interface was proposed.
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