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Reversible conduction block in isolated toad sciatic nerve by emulsified isoflurane.

Zhuo Li¹, Jing Yang, Jin Liu

¹Laboratory of Anesthesia and Critical Care Medicine, State Key Laboratory of Biotherapy of Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. weiweiyang72@gmail.com

Anesthesia and Analgesia

|April 2, 2010

Summary

Emulsified isoflurane (EI) demonstrated reversible, dose-dependent nerve conduction block in toad sciatic nerves. EI showed slower onset but faster recovery compared to lidocaine.

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Area of Science:

Neuroscience
Pharmacology
Anesthesiology

Background:

Volatile anesthetics can exhibit local anesthetic properties.
Emulsified isoflurane (EI) is a novel formulation for potential local anesthetic use.

Purpose of the Study:

To evaluate the nerve conduction block characteristics of emulsified isoflurane (EI).
To compare the nerve blockade of EI with 1% lidocaine.

Main Methods:

Isolated toad sciatic nerves were exposed to varying concentrations of EI, lidocaine, Intralipid, or Ringer solution.
Compound nerve action potential (CNAP) parameters were measured using extracellular recording.
Nerve block reversibility was assessed after washing with Ringer solution.

Main Results:

Both EI and lidocaine decreased CNAP amplitudes and conduction velocities.
EI exhibited a dose-dependent effect with a half maximal inhibitory concentration of 5.46%.
EI showed a slower onset (7 min) and faster recovery (9 min) compared to lidocaine (1 min onset, 30 min recovery).

Conclusions:

Emulsified isoflurane induces reversible, dose-dependent nerve conduction inhibition.
EI offers a distinct kinetic profile with slower onset and quicker recovery than lidocaine.