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Published on: December 19, 2020
Protective potential of an attenuated Pasteurella multocida, which expresses only the N-terminal truncated fragment
Jayoung Seo1, Semi Lee, Hyoju Pyo
1Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Abstract:
Pasteurella multocida serogroup D causes progressive atrophic rhinitis in pigs and produces a potent, intracellular, mitogenic toxin known as P. multocida toxin (PMT), which is encoded by the toxA gene. Highly toxic to cells, PMT is a poor antigen and becomes more immunogenic after its native structure has been destroyed. Previously, we found that the N-terminal fragment of PMT (N-PMT) can induce a strong immune response that is protective against wild-type challenge. Here, an attenuated P. multocida mutant expressing only N-PMT was developed and its protective effect was evaluated. The mutant provides protective immune responses against bacterial and toxin challenges, and so is a good live vaccine candidate.
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