Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood circulating C228T TERT gene promoter mutation is an independent factor for the prognosis of HBV-related hepatocellular carcinoma.

BMC cancer·2026
Same author

Incorporating real-world scenarios during initial validation stages of point-of-care human papillomavirus (HPV) screening tests: a scoping review.

Sexually transmitted infections·2026
Same author

Auto-SELEX: a fully automated microfluidic platform for rapid discovery of high-affinity aptamers.

Lab on a chip·2026
Same author

Systematic review of point-of-need molecular diagnostics for rotavirus and enteric adenoviruses F40/F41.

BMC infectious diseases·2026
Same author

One-pot CRISPR-based point of care platform for rapid, specific and sensitive detection of HPV 16 without pre-amplification.

Microsystems & nanoengineering·2026
Same author

Lateral flow biosensors for low abundance detection of brain natriuretic peptide with enzyme-free amplification.

Lab on a chip·2026

Related Experiment Video

Updated: Jun 14, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Lateral patch-clamping in a standard 1536-well microplate format.

Kum Cheong Tang1, Julien Reboud, Yuan Li Kwok

  • 1Institute of Microelectronics (IME), 11 Science Park Road, Science Park II, Singapore.

Lab on a Chip
|April 2, 2010
PubMed
Summary

This study scales up lateral patch-clamping, a chip-based method for studying cellular ion channels, to a 1536-well format. This advancement enables high-throughput screening of compounds, overcoming limitations of the conventional patch-clamp technique.

More Related Videos

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Related Experiment Videos

Last Updated: Jun 14, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Area of Science:

  • Biophysics
  • Microfluidics
  • Cellular Biology

Background:

  • Conventional patch-clamp is the gold standard for ion channel study but is low-throughput and labor-intensive.
  • Automated chip-based platforms are needed to overcome the limitations of manual patch-clamping for drug screening.

Purpose of the Study:

  • To scale up lateral patch-clamping technology to a 1536-well microtiter plate format.
  • To enable high-throughput automated cell patching and ion channel recording.

Main Methods:

  • Fabrication of a 1536-well microtiter plate unit on a microstructured silicon substrate with integrated glass capillaries and PDMS capping.
  • Development of a microfluidic network for cell and buffer delivery.
  • Testing the system using RBL-1 cells to record whole-cell activity of endogenous potassium channels.

Main Results:

  • Successful integration of 16 lateral junction wells on a 9 mm x 9 mm silicon chip.
  • Demonstration of automated cell trapping and patching using the microfluidic system.
  • Recording of whole-cell ion channel activity with a revised protocol to ensure accurate ionic composition.

Conclusions:

  • The developed 1536-well lateral patch-clamp system significantly enhances throughput for ion channel research.
  • This scalable platform facilitates automated cellular assays and compound screening.
  • Optimized protocols are crucial for reliable electrophysiological recordings in microfluidic devices.