Related Experiment Video
Updated: Jun 14, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
Expression of Vitreoscilla hemoglobin enhances cell growth and dihydroxyacetone production in Gluconobacter oxydans
Minghua Li1, Jian Wu, Jinping Lin
1State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, Shanghai, China. limh09@hotmail.com
Abstract:
Dihydroxyacetone (DHA) is an important ketose sugar, which is extensively used in the cosmetic, chemical, and pharmaceutical industries. DHA has been industrially produced by Gluconobacter oxydans with a high demand of oxygen. To improve the production of DHA, the gene vgb encoding Vitreoscilla hemoglobin (VHb) was successfully introduced into G. oxydans, where it was stably maintained, and expressed at about 76.0 nmol/g dry cell weight. Results indicated that the constitutively expressed VHb improved cell growth and DHA production in G. oxydans under different aeration conditions. Especially at low aeration rates, the VHb-expressing strain (VHb(+)) displayed 23.13% more biomass and 37.36% more DHA production than those of VHb-free strain (VHb(-)) after 32 h fermentation in bioreactors. In addition, oxygen uptake rate (OUR) was also increased in VHb(+) strain relative to the control strain during fermentation processes.
More Related Videos
Related Concept Videos
Hyperthermophilic Bacteria
Microbial Growth Media
Biosynthesis in Bacteria
Bacterial Signaling
Bacterial Growth Curve
Bioreactor Controls-III

