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Updated: Jun 14, 2026
Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Published on: September 19, 2018
Antibody array-based technologies for cancer protein profiling and functional proteomic analyses using serum and
1Tumor Markers Group, Spanish National Cancer Research Center, 310A, Melchor Fernandez Almagro 3, 28029, Madrid, Spain. mscarbayo@cnio.es
Abstract:
In the context of other proteomic technologies, targeted antibody arrays are strongly contributing for protein profiling and functional proteomics analyses in serum specimens. Protein-protein interactions, post-translational modifications, and interaction between protein and DNA or RNA can all shift the activity of a protein from what would have been predicted by its level of transcription. Functional proteomics studies the interaction of proteins within their cellular environment to determine how a given protein accomplishes its specific cellular task. Accordingly, the promise of protein profiling and functional proteomics is that by chronicling the function of aberrant or over-expressed proteins, it will be possible to characterize the mechanism of the disease-sustaining proteins. The further understanding of the disease networks will eventually lead to targeted cancer therapy and specific biomarkers for diagnosis, prognosis or therapeutic response prediction based on disease specific proteins. This review describes how such strategies reported to date in serum specimens may assist in characterizing tumor biology, and for the diagnosis, surveillance, prognosis, and potentially for predictive and therapeutic purposes for patients affected with solid and hematological neoplasias.
Insights
Targeted antibody arrays enable protein profiling and functional proteomics in serum. This approach aids in understanding disease mechanisms, leading to targeted cancer therapies and diagnostic biomarkers.
Area of Science:
- Proteomics
- Biochemistry
- Molecular Biology
Background:
- Protein profiling and functional proteomics are crucial for understanding cellular functions and disease mechanisms.
- Protein activity is influenced by interactions, post-translational modifications, and nucleic acid binding, not just transcription levels.
- Aberrant or over-expressed proteins are key to characterizing disease-sustaining mechanisms.
Purpose of the Study:
- To review the application of targeted antibody arrays in serum for protein profiling and functional proteomics.
- To highlight the potential of these strategies in characterizing tumor biology.
- To explore their utility in cancer diagnosis, prognosis, surveillance, and therapeutic response prediction.
Main Methods:
- Utilizes targeted antibody arrays for protein profiling in serum specimens.
- Focuses on analyzing protein-protein interactions, post-translational modifications, and protein-nucleic acid interactions.
- Reviews existing literature on strategies applied to serum samples.
Main Results:
- Targeted antibody arrays offer a powerful tool for detailed protein analysis in serum.
- These methods can elucidate disease-specific protein functions and interactions.
- The review consolidates findings on the application of these strategies in various cancer types.
Conclusions:
- Protein profiling and functional proteomics using targeted antibody arrays in serum are vital for advancing cancer research.
- Understanding disease-specific proteins can lead to the development of targeted cancer therapies.
- These approaches hold significant promise for improving cancer diagnosis, prognosis, and treatment strategies.
