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Bacterial attachment on optical fibre surfaces.

N Mitik-Dineva1, J Wang, V K Truong

  • 1Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia.

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Chemically etched optical fibers prevent bacterial attachment by altering surface properties. Unetched fibers, however, show varied bacterial adhesion, highlighting the importance of surface modification for biosensor applications.

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Microbiology

Background:

  • Optical fibers are explored as sensor arrays for biomolecule detection.
  • Surface modification is key to controlling biological interactions on optical fibers.
  • Understanding bacterial adhesion is crucial for biosensor development and preventing contamination.

Purpose of the Study:

  • To investigate the effect of chemical etching on optical fiber surface properties.
  • To evaluate bacterial attachment to both etched and non-etched optical fiber surfaces.
  • To determine the influence of surface topography and chemistry on bacterial adhesion.

Main Methods:

  • Chemical etching to create micro-scale wells on optical fiber surfaces.
  • Sessile pico-drop method for surface hydrophobicity analysis (water contact angle).
  • Atomic Force Microscopy (AFM) for surface topography characterization.
  • Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) for surface chemistry analysis.
  • Bacterial attachment assays using six different bacterial strains.

Main Results:

  • Etched optical fibers exhibited a lower water contact angle (96° ± 10°) and were not amenable to bacterial attachment.
  • Non-etched optical fibers had a higher water contact angle (106° ± 4°) and a smoother nano-scale surface (Rq = 273 nm), promoting bacterial adhesion.
  • Bacterial attachment varied among different strains on the non-etched fiber surfaces.
  • Surface topography and hydrophobicity significantly influenced bacterial adhesion.

Conclusions:

  • Chemical etching effectively modifies optical fiber surfaces to prevent bacterial attachment.
  • Surface properties, including topography and hydrophobicity, play a critical role in bacterial adhesion.
  • Tailoring optical fiber surface chemistry is essential for developing robust biosensors and controlling biofouling.