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Updated: Jun 14, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica
Published on: December 12, 2010
Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants
C Rune Stensvold1, Marianne Lebbad, C Graham Clark
1Laboratory of Parasitology, Statens Serum Institut, Copenhagen, Denmark. run@ssi.dk
Abstract:
Six ssrRNA gene sequences were obtained by PCR amplification of DNA from uninucleated Entamoeba cysts isolated from fresh faeces of sheep, cows, a roe deer and a reindeer. Phylogenetic analysis using sequences of non-, uni-, quadri- and octonucleate cyst-producing Entamoeba spp. for comparison showed that all six isolates formed a separate clade nested within the clade of quadrinucleate cyst producers. The data indicate that Entamoeba bovis can be isolated from ruminant hosts other than cattle, and we suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis.
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