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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
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Related Experiment Video

Updated: Jun 14, 2026

Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique

Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique

Published on: December 12, 2025

Optimized DNA preparation from mycobacteria.

Michael Käser¹, Marie-Thérèse Ruf, Julia Hauser

¹Ghanaian-German Centre for Health Research, University of Ghana, School of Public Health, Legon, Accra, Ghana. m.kaeser@unibas.ch

Cold Spring Harbor Protocols

|April 3, 2010

Summary

This study presents a new method for extracting genomic DNA from mycobacteria, overcoming challenges of slow growth and tough cell walls. The optimized protocol significantly increases DNA yield and purity for advanced genomic analyses.

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Last Updated: Jun 14, 2026

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Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Mycobacterial DNA extraction is challenging due to slow growth and robust cell walls, often yielding insufficient DNA quantity and quality.
Existing methods and kits are primarily for PCR-based applications, not advanced genomic analyses like whole-genome sequencing.
High-yield protocols often use large volumes, increasing costs and waste.

Purpose of the Study:

To develop an improved DNA extraction method for mycobacteria that enhances yield and purity.
To address the limitations of existing protocols in terms of DNA quantity, quality, and cost-effectiveness.
To provide a practical and economical method suitable for contemporary genomic research.

Main Methods:

A novel DNA extraction protocol optimized for mycobacteria.
Utilizes small-volume (1.5-mL tubes) processing for efficiency.
Focuses on overcoming cell wall lysis and slow growth challenges.

Main Results:

Achieved significantly higher yields of pure genomic DNA compared to previous methods.
The new protocol is economical and practical, requiring minimal equipment and chemicals.
Yield increases of at least 10-fold were observed.

Conclusions:

The developed method reliably produces large amounts of pure mycobacterial genomic DNA.
This protocol is suitable for advanced genomic applications requiring high-quality DNA.
Offers a cost-effective and efficient solution for mycobacterial DNA isolation.