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Related Experiment Video

Updated: Jun 14, 2026

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
08:32

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Published on: July 13, 2017

rAAV9--a human-derived adeno-associated virus vector for efficient transgene expression in mouse cingulate cortex.

Mira Jakovcevski1, Yin Guo, Qin Su

  • 1Department of Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, Worcester, MA 01604, USA. Mira.Jakovcevski@umassmed.edu

Cold Spring Harbor Protocols
|April 3, 2010
PubMed
Summary

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Researchers developed an efficient gene delivery method for the juvenile mouse prefrontal cortex (PFC) using adeno-associated virus serotype 9 (AAV9). This new system enables long-lasting gene expression, crucial for studying neurodevelopmental diseases.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The rodent rostro-medial cortex is a model for primate prefrontal cortex (PFC).
  • Understanding PFC development is key for neurodevelopmental diseases like schizophrenia.
  • Efficient gene delivery in juvenile PFC is currently lacking.

Purpose of the Study:

  • To establish an effective gene delivery system for the juvenile mouse PFC.
  • To investigate molecular mechanisms during critical PFC developmental stages.
  • To overcome limitations of existing gene delivery vectors in the PFC.

Main Methods:

  • Utilized adeno-associated virus (AAV) serotype 9 for gene delivery.
  • Targeted gene delivery in juvenile (postnatal day 21) and young adult mice.

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  • Assessed transgene expression in the PFC.

    • Main Results:

    • Developed a protocol for efficient AAV9-mediated gene delivery in the mouse PFC.
    • Achieved long-lasting transgene expression in both juvenile and young adult PFC.
    • Demonstrated AAV9's superiority over AAV2 for broader transduction in the PFC.

    Conclusions:

    • AAV9 provides an efficient and long-lasting gene delivery method for the juvenile and young adult mouse PFC.
    • This system facilitates preclinical research on neurodevelopmental disorders affecting the PFC.
    • The protocol overcomes limitations of previous methods for PFC gene expression.