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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Jun 14, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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Published on: November 9, 2020

Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.

Jikai Wen1, Saverio Brogna

  • 1School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK.

The EMBO Journal
|April 3, 2010
PubMed
Summary

Nonsense-mediated mRNA decay (NMD) enhances mRNA surveillance. In fission yeast, intron proximity to premature termination codons, not exon junction complexes, dictates NMD efficiency, challenging existing models.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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Published on: November 9, 2020

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Nonsense-mediated mRNA decay (NMD) is a crucial surveillance pathway eliminating aberrant mRNAs with premature translation termination codons (PTCs).
  • In mammals, NMD is coupled to pre-mRNA splicing, with PTCs typically triggering NMD only when upstream of an intron, a process often mediated by the exon junction complex (EJC).

Purpose of the Study:

  • To investigate the relationship between pre-mRNA splicing and NMD in the fission yeast Schizosaccharomyces pombe.
  • To determine the role of intron-exon boundaries and EJC components in splicing-enhanced NMD in this organism.

Main Methods:

  • Utilized genetic manipulation in Schizosaccharomyces pombe to create specific intron-PTC configurations.
  • Assessed NMD efficiency through mRNA quantification techniques.

Main Results:

  • Splicing enhances NMD in Schizosaccharomyces pombe, irrespective of intron position relative to the PTC (upstream or downstream).
  • The exon junction complex (EJC) is not required for splicing-mediated NMD enhancement in this yeast model.
  • NMD efficiency is directly correlated with the proximity of an intron to the PTC, rather than merely the occurrence of splicing.

Conclusions:

  • The mechanism linking splicing and NMD in Schizosaccharomyces pombe differs from the mammalian EJC-dependent model.
  • Intron proximity to the PTC is a key determinant of splicing-enhanced NMD.
  • A novel model for splicing-NMD interplay is proposed based on these findings.