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Published on: December 5, 2019
Histological study of fresh versus frozen semitendinous muscle tendon allografts
Alexandre Carneiro Bitar1, Luiz Augusto Ubirajara Santos, Alberto Tesconi Croci
1Institute of Orthopedics and Traumatology, Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP, Brazil.
Objective:
The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80 degrees C in comparison to a control group kept at only -4 degrees C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures.
Methods:
In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80 degress C as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh".
Results:
There was no histological difference between the fresh and frozen samples in relation to seven variables.
Conclusions:
Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80 degrees C without suffering histological modifications.
