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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 14, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

[Confocal laser scanning microscopy].

S Astner1, M Ulrich

  • 1Abteilung für Dermatologie, Venerologie und Allergologie, Charité Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Deutschland.

Der Hautarzt; Zeitschrift Fur Dermatologie, Venerologie, Und Verwandte Gebiete
|April 3, 2010
PubMed
Summary
This summary is machine-generated.

Confocal laser scanning microscopy (LSM) offers a non-invasive method for diagnosing skin conditions, reducing the need for painful biopsies. This technique aids in evaluating dynamic skin processes and treatment responses.

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Last Updated: Jun 14, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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Published on: October 20, 2011

Area of Science:

  • Dermatology
  • Optical Diagnostics
  • Medical Imaging

Context:

  • Traditional skin disease diagnosis relies on clinical inspection and invasive histology.
  • Histology involves tissue removal, posing risks of pain, infection, and scarring.
  • Non-invasive diagnostic devices are increasingly evaluated in dermatology.

Purpose:

  • To assess the applicability of confocal laser scanning microscopy (LSM) as a non-invasive diagnostic tool in dermatology.
  • To correlate LSM images with histological features for defining diagnostic parameters.
  • To establish LSM as an adjunct diagnostic device for specific skin conditions.

Summary:

  • Confocal laser scanning microscopy (LSM) images were correlated with routine histology findings.
  • Diagnostic LSM parameters were defined for selected skin diseases.
  • LSM proved effective as an adjunct diagnostic device in clinical and investigative dermatology.

Impact:

  • LSM provides a non-invasive alternative to skin biopsies, minimizing patient discomfort and risks.
  • Repeated LSM examinations allow for monitoring dynamic skin processes, including neoplastic and regenerative changes.
  • LSM facilitates the assessment of disease extent and response to therapy, improving patient management.