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Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time

Xing Ye1, Lili Zhang, Haiyan Dong

  • 1Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation, CAFS, Guangzhou 510380, People's Republic of China. gzyexing@163.com

Biotechnology Letters
|April 3, 2010
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...