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Astaxanthin addition improves human neutrophils function: in vitro study.

Rita C Macedo1, Anaysa P Bolin, Douglas P Marin

  • 1Postgraduate Program, Health Science, CBS, Cruzeiro do Sul University, Avenida Regente Feijó, 1295. Tatuapé, São Paulo, SP CEP 03342-000, Brazil.

European Journal of Nutrition
|April 3, 2010
PubMed
Summary
This summary is machine-generated.

Astaxanthin (ASTA) enhances human neutrophil function, boosting their ability to engulf and kill microbes. This antioxidant effect reduces harmful oxidative stress and inflammation markers.

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Area of Science:

  • Immunology
  • Cell Biology
  • Nutritional Science

Background:

  • Neutrophils are critical immune cells involved in pathogen defense.
  • Oxidative stress can impair neutrophil function.
  • Astaxanthin is a potent antioxidant carotenoid.

Purpose of the Study:

  • To investigate the in vitro effects of astaxanthin on human neutrophil functions.
  • To assess astaxanthin's impact on phagocytic and microbicidal capacities.
  • To evaluate astaxanthin's influence on cytokine release and reactive oxygen species (ROS) production.

Main Methods:

  • Human neutrophils were treated with astaxanthin (ASTA).
  • Assays included phagocytosis, microbicidal capacity, intracellular calcium, ROS (superoxide anion, hydrogen peroxide, nitric oxide), antioxidant enzyme activity, oxidative damage markers, and cytokine release (IL-6, TNF-alpha).

Main Results:

  • Astaxanthin significantly enhanced neutrophil phagocytic and microbicidal capacity.
  • ASTA increased intracellular calcium and nitric oxide (NO·) production.
  • ASTA treatment decreased superoxide anion, hydrogen peroxide, IL-6, and TNF-α production, alongside reduced oxidative damage to lipids and proteins.

Conclusions:

  • Astaxanthin demonstrates a beneficial effect on human neutrophil function.
  • Enhanced phagocytic and fungicidal capacity is observed with ASTA treatment.
  • The mechanism involves calcium mobilization and nitric oxide production, with reduced oxidative stress markers.