Related Experiment Video
Updated: Jun 14, 2026
07:21
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Published on: July 29, 2018
Derivation of multipotent progenitors from human circulating CD14+ monocytes
Noriyuki Seta1, Masataka Kuwana
1Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Experimental Hematology
|April 6, 2010
Summary
Circulating monocytes are more versatile than previously believed, giving rise to multipotent cells (MOMCs) capable of tissue repair and regeneration. These findings expand our understanding of monocyte functions beyond immunity.
Area of Science:
- * Stem Cell Biology
- * Immunology
- * Regenerative Medicine
Background:
- * Circulating CD14(+) monocytes are considered precursors for phagocytes like macrophages.
- * A novel primitive cell population, monocyte-derived multipotential cells (MOMCs), was recently identified.
- * The circulating precursors for MOMCs remained undetermined.
Purpose of the Study:
- * To identify the circulating precursors for MOMCs.
- * To characterize the differentiation potential of MOMCs.
- * To explore the role of MOMCs in tissue repair and hematopoiesis.
Main Methods:
- * Comparative gene expression profiling of MOMCs and other monocyte-derived cells.
- * In vitro culture of MOMCs requiring fibronectin and platelet-derived factors.
- * Analysis of MOMC differentiation capacity into various non-phagocytic cell types.
- * Assessment of MOMCs' ability to promote hematopoietic progenitor cell expansion.
Main Results:
- * MOMCs express embryonic stem cell markers Nanog and Oct-4.
- * MOMCs differentiate into diverse cell types including bone, cartilage, muscle, neurons, and endothelium.
- * MOMCs support ex vivo expansion of hematopoietic progenitor cells.
- * Circulating monocytes are more multipotent than previously recognized.
Conclusions:
- * Circulating CD14(+) monocytes can differentiate into MOMCs, which possess multipotent progenitor capacity.
- * MOMCs contribute to tissue repair and regeneration beyond immune responses.
- * Monocytes play a broader role in physiology, including tissue repair and hematopoiesis.
Related Concept Videos
01:15Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
01:21Lineage Commitment
Commitment is the process whereby stem cells:
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:34Production of Formed Elements
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Most HSCs commit to...