Related Experiment Video
Updated: Jun 14, 2026
11:20
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
Published on: October 16, 2014
Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
Hiroyuki Kamiya1, Hitomi Goto, Genki Kanda
1Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan. hirokam@pharm.hokudai.ac.jp
International Journal of Pharmaceutics
|April 6, 2010
Summary
Histone-mediated gene delivery using exogenous histone H3 complexed with plasmid DNA was studied. Results show histone H3 suppresses transgene expression, indicating this method is unlikely to be useful for gene delivery.
Area of Science:
- Molecular Biology
- Gene Delivery
- Cell Biology
Background:
- Intranuclear disposition of plasmid DNA is crucial for effective transgene expression.
- Exogenous histones have been explored as carriers for plasmid DNA in gene delivery systems.
Purpose of the Study:
- To investigate the impact of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency.
- To evaluate the feasibility of histone-mediated gene delivery based on intranuclear disposition.
Main Methods:
- Plasmid DNA was complexed with exogenous histone H3 in various ratios.
- Complexes were transfected into HeLa cells using osmotic pressure.
- Transgene expression efficiency was measured.
Main Results:
- Histone H3 significantly suppressed transgene expression within the nucleus.
- This suppression effect was observed in a dose-dependent manner.
- The intranuclear disposition of histone H3-plasmid DNA complexes negatively affected gene expression.
Conclusions:
- Histone-mediated gene delivery using histone H3 is likely ineffective due to its suppressive effect on transgene expression.
- The intranuclear disposition of histone H3-plasmid DNA complexes poses a significant challenge for successful gene delivery.
