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Updated: Jun 14, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
2-C-methyluridine modified hammerhead ribozyme against the estrogen receptor
Rodrigo Pontiggia1, Osvaldo Pontiggia, Marina Simian
1INGEBI (CONICET),Vuelta de Obligado 2490-(1428), Buenos Aires, Argentina.
Abstract:
A new synthesis of 2'-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5'-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor alpha protein (ER-alpha), and transfection experiments in MCF-7 cell line are also presented.
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