Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

DNA methylation detection using MS-qFRET, a quantum dot-based nanoassay.

Vasudev J Bailey1, Brian P Keeley, Christopher R Razavi

  • 1Department of Biomedical Engineering, Johns Hopkins School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, United States.

Methods (San Diego, Calif.)
|April 6, 2010
PubMed
Summary

This study introduces Methylation specific quantum dot fluorescence resonance energy transfer (MS-qFRET), a sensitive nanotechnology assay for detecting DNA methylation changes. The assay was validated and used to analyze p15(INK4B) methylation in acute myeloid leukemia patient samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal analysis of tyrosine kinase inhibitor therapy outcomes and BCR-ABL1 transcript decline velocity in chronic myeloid leukemia: A 16-year real-world study.

Cancer·2026
Same author

Cytokine co-presentation on targeted lipid nanoparticles enhances in vivo T cell engineering.

Biomaterials·2026
Same author

Outcomes of patients with higher-risk myelodysplastic syndromes/neoplasms treated with hypomethylating agents + venetoclax-an analysis from the International Consortium for MDS (icMDS) VALIDATE database.

Blood cancer journal·2026
Same author

Real-world, multi-omics validation of the clinical relevance of molecular taxonomy for myelodysplastic syndromes (MDS).

HemaSphere·2026
Same author

Prevalence of HIV and associated sociobehavioral factors among men with urethral discharge syndrome attending public health facilities in Kampala, Uganda.

BMC infectious diseases·2026
Same author

Cytokine receptor and JAK/STAT pathway mutations in acute myeloid leukemia: Prevalence and clinical impact.

Cancer·2026

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Aberrant promoter hypermethylation of tumor suppressor genes serves as a prognostic marker in carcinogenesis.
  • Epigenetic modifying agents are FDA-approved for treating myelodysplastic syndrome, necessitating tools to monitor therapy response.
  • Current methods for detecting DNA methylation require improvement in sensitivity and quantifiability.

Purpose of the Study:

  • To validate the Methylation specific quantum dot fluorescence resonance energy transfer (MS-qFRET) assay for DNA methylation detection.
  • To apply the MS-qFRET assay for analyzing p15(INK4B) methylation status in clinical samples.
  • To establish a sensitive and quantifiable laboratory tool for monitoring epigenetic modifications.

Main Methods:

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Related Experiment Videos

Last Updated: Jun 14, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

  • DNA methylation detection using quantum dot-mediated fluorescence resonance energy transfer (MS-qFRET).
  • Sodium bisulfite treatment of template DNA to differentiate methylated and unmethylated cytosines.
  • Biotinylated methylation-specific primer amplification and quantum dot-streptavidin conjugation for FRET detection.

    • Main Results:

    • The MS-qFRET assay demonstrated sensitive and quantifiable detection of DNA methylation.
    • The assay was successfully validated in cell line experiments.
    • p15(INK4B) methylation status was successfully detected in clinical samples from acute myeloid leukemia patients.

    Conclusions:

    • MS-qFRET is a validated, sensitive, and quantifiable nanotechnology assay for detecting DNA methylation.
    • This assay can be utilized as a laboratory correlative tool to monitor responses to epigenetic therapies.
    • MS-qFRET holds potential for analyzing gene methylation in clinical settings, including cancer diagnostics and prognostics.