Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution of Adaptive Non-Shivering Thermogenesis in Mammals.

Physiology (Bethesda, Md.)·2026
Same author

Retention and tissue-specific expression of uncoupling protein homoeologs in Xenopus laevis.

Biology open·2026
Same author

Considerations and best practices for respirometry.

Cell metabolism·2026
Same author

Publisher Correction: GLP-1R-GIPR-PPARα/γ/δ quintuple agonism corrects obesity and diabetes in mice.

Nature·2026
Same author

Why Physiology Needs to Embrace Structural Biology - The Hot Case of Thermogenic UCP1.

Acta physiologica (Oxford, England)·2026
Same author

GLP-1R-GIPR-PPARα/γ/δ quintuple agonism corrects obesity and diabetes in mice.

Nature·2026

Related Experiment Video

Updated: Jun 14, 2026

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Mitochondrial uncoupling and lifespan.

Shona A Mookerjee1, Ajit S Divakaruni, Martin Jastroch

  • 1Buck Institute for Age Research, Novato, CA 94945 USA. smookerj@buckinstitute.org

Mechanisms of Ageing and Development
|April 6, 2010
PubMed
Summary
This summary is machine-generated.

Mitochondrial uncoupling, a process affecting metabolic rate and reactive oxygen species (ROS), is explored for its role in aging. This review examines how mitochondrial uncoupling impacts lifespan extension, though its consistent role remains unclear.

More Related Videos

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
06:53

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Published on: November 23, 2011

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
09:47

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Published on: June 2, 2023

Related Experiment Videos

Last Updated: Jun 14, 2026

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
06:53

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Published on: November 23, 2011

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
09:47

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Published on: June 2, 2023

Area of Science:

  • Gerontology and cellular metabolism.
  • Mitochondrial biology and aging research.

Background:

  • Aging research increasingly focuses on metabolic control, particularly mitochondrial activity.
  • Mitochondria regulate energy production (ATP), reactive oxygen species (ROS), and heat, influencing cellular health.
  • Mitochondrial inefficiency via proton leak, uncoupling substrate oxidation from ATP production, can significantly impact metabolic rate.

Purpose of the Study:

  • To review recent research on the impact of mitochondrial uncoupling on lifespan.
  • To explore the mechanisms by which mitochondrial uncoupling may influence aging processes.

Main Methods:

  • Review of existing scientific literature on mitochondrial uncoupling and lifespan.
  • Analysis of studies investigating the relationship between metabolic rate, ROS production, and longevity.

Main Results:

  • Mitochondrial uncoupling can affect metabolic rate and reduce ROS production, factors implicated in aging.
  • Pharmacological induction of proton leak and uncoupling proteins are methods studied.
  • Current evidence does not consistently link mitochondrial uncoupling to lifespan extension.

Conclusions:

  • Mitochondrial uncoupling is a key area in aging research due to its metabolic effects.
  • While theoretically linked to longevity, a definitive role for uncoupling in extending lifespan requires further investigation.