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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
Published on: May 28, 2021
The nuclear matrix shell proteome of human epidermis
Raymond L Warters1, Pamela B Cassidy, Jeremy A Sunseri
1Department of Radiation Oncology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States.
Journal of Dermatological Science
|April 6, 2010
Summary
Researchers successfully isolated nuclear matrix proteins from intact human skin, identifying distinct protein profiles in epidermis versus cultured skin cancer cells. This method may reveal novel melanoma biomarkers.
Area of Science:
- Proteomics
- Cell Biology
- Dermatology
Background:
- Proteomic analysis has identified cancer-specific biomarkers in nuclear matrix fractions of cancer cells.
- Investigating melanoma biomarkers using similar proteomic approaches was explored.
Purpose of the Study:
- To determine if a nuclear matrix fraction can be isolated from intact human epidermis.
- To compare the proteome of cultured melanoma and carcinoma cells with that of intact epidermis.
Main Methods:
- Two-dimensional electrophoresis (2DGE) and LC/MS/MS were used.
- Proteins were identified in the nuclear matrix shell fraction from human epidermis and cultured skin/cancer cells.
Main Results:
- Nuclear matrix shell isolation from intact epidermis yielded ~40% of total protein.
- Cultured skin cells yielded only 5-10% in the nuclear matrix shell.
- Epidermal nuclear matrix differed from cultured cells by keratin 1, vimentin, and desmoplakin expression.
Conclusions:
- The nuclear matrix-intermediate filament system is isolatable from intact human epidermis.
- Analyzing this fraction's protein composition may identify cancer-specific biomarkers for skin cancers.
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