Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

Chromosome Replication

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

DNA Replication

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transcription at an inducible common fragile site reveals replication origin strength hierarchy.

Nucleic acids research·2026

Same author

Dynamic forces shape the survival fate of eliminated cells.

Nature physics·2025

Same author

Histone H3 serine-57 is a CHK1 substrate whose phosphorylation affects DNA repair.

Nature communications·2023

Same author

Dimeric G-quadruplex motifs-induced NFRs determine strong replication origins in vertebrates.

Nature communications·2023

Same author

Firing of Replication Origins Is Disturbed by a CDK4/6 Inhibitor in a pRb-Independent Manner.

International journal of molecular sciences·2023

Same author

Promoters are key organizers of the duplication of vertebrate genomes.

BioEssays : news and reviews in molecular, cellular and developmental biology·2021

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Interplay between DNA replication and gene expression: a harmonious coexistence.

Chrystelle Maric¹, Marie-Noëlle Prioleau

¹Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, Paris, France.

Current Opinion in Cell Biology

|April 6, 2010

Summary

This summary is machine-generated.

Genomic DNA replication and gene expression are crucial for multicellular organisms but not strictly co-regulated. Highly transcribed genes can impede DNA replication, highlighting the need for further research into their complex interplay.

More Related Videos

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jun 14, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

Molecular Biology
Genomics
Cell Biology

Background:

Multicellular organisms require precise genome duplication and gene expression for proper function.
Sophisticated molecular machinery governs these fundamental cellular processes.
Recent large-scale studies investigate the interaction between DNA replication and gene expression.

Purpose of the Study:

To elucidate the complex interplay between DNA replication and gene expression in multicellular organisms.
To understand how these fundamental processes, despite sharing regulatory elements, are not strictly co-regulated.

Main Methods:

Analysis of large-scale genomic studies.
Investigation of the replisome's interaction with transcribed genes.
Examination of cis-regulatory elements involved in replication and transcription.

Main Results:

The machinery for genome duplication and gene expression shares similar cis-regulatory elements but is not tightly co-regulated.
Highly transcribed genes pose a significant obstacle to the proper functioning of the replisome.
The spatio-temporal program of DNA replication is influenced by the interplay with transcription.

Conclusions:

The relationship between DNA replication and transcription is complex and not fully understood.
Further research is essential to identify key regulators governing the spatio-temporal program of DNA replication.
Elucidating the intricate interplay between replication and transcription is crucial for understanding genome stability and gene expression.