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Published on: June 6, 2017
Mitotic cell-cycle progression is regulated by CPEB1 and CPEB4-dependent translational control
Isabel Novoa1, Javier Gallego, Pedro G Ferreira
1Gene Regulation Program, Centre for Genomic Regulation (CRG), 08003 Barcelona, Spain. inovoa@ir.vhebron.net
Nature Cell Biology
|April 6, 2010
Summary
Cytoplasmic polyadenylation and CPEB proteins regulate gene expression by controlling mRNA poly(A) tail length. This mechanism is crucial for cell proliferation and entry into mitosis in dividing cells.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Meiotic and early embryonic cell divisions rely on translational regulation of maternal mRNAs due to lack of transcription.
- Cytoplasmic-polyadenylation-element-binding protein (CPEB) controls mRNA translation via poly(A) tail length modification.
Purpose of the Study:
- To investigate if CPEB-mediated translational regulation impacts mitotic cell cycle divisions.
- To determine the role of poly(A) tail length changes in cell proliferation and mitosis.
Main Methods:
- Analysis of CPEB function in mitotically dividing cells.
- Investigating the impact of CPEB1 and CPEB4 on cell proliferation and cell cycle progression.
Main Results:
- CPEB-mediated post-transcriptional regulation via poly(A) tail length changes is essential for cell proliferation.
- This translational control is specifically required for the entry into M phase of mitosis.
- CPEB1 and CPEB4 were identified as key mediators of this process.
Conclusions:
- Poly(A) tail length regulation by CPEB is a general mechanism controlling mitosis.
- This mechanism compensates for lack of transcription not only in specialized divisions but also in general cell proliferation.
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