Related Experiment Video
Updated: Jun 14, 2026
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
Published on: January 7, 2018
[Effect of compound coarse grain food on insulin resistance and expression of PPARgamma in rat]
Zhoubo Ding1, Xiaofei Wang, Chengkai Zhai
1School of Public Health, Southeast University, Nanjing 210009, China. dingzhoubo@yahoo.com.cn
Objective:
To explore the effect of compound coarse grain food on insulin resistance (IR) in rats.
Methods:
Forty Sprague-Dawley rats were randomly divided into normal control group (n=10) and model group (n=30), provided regular diet and high fat diet separately. After 6 weeks, the model group were divided into high fat diet group, rice flour group and coarse grain group, and provided the corresponding feed. After feeding 9 weeks, the fasting blood glucose, insulin and the expression of PPAR-gamma mRNA in rats were measured.
Results:
IR was successfully induced by 6-weeks high fat diet. The body weight and insulin resistance index (HOMA-IR) in coarse grain group were significantly lower than high fat diet group and rice flour group. The expression of PPAR-gamma mRNA in coarse grain group was significantly higher than other three groups (P < 0.05).
Conclusion:
The coarse grain food can reduce the concentration of blood glucose and insulin in rat with insulin resistance induced by high-fat dietary, and improve insulin sensitivity. The possible mechanism is the over-expression of PPAR-gamma.
Related Concept Videos
01:24Glucagon-like Receptor Agonists
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by the...
01:28Insulin: The Receptor and Signaling Pathways