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Updated: Jun 14, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
[Effect of Arg-Gly-Asp peptide on the secretion of vascular endothelial growth factor from vascular endothelial
Qing Guan1, Zhongyan Li, Soban Uumar
1School of Nursing, Jilin Medical College, Jilin 132013, China. georgina27@sina.com
Objective:
To investigate the effect of Arg-Gly-Asp peptides (RGD peptides)on the secretion of vascular endothelial growth factor (VEGF) from vascular endothelial cells (VEC) and the mechanism.
Methods:
Using VEC cultured from the arteries of human greater omentum as a model, and detecting the content of VEGF and the activity of protein kinase C (PKC) under different concentration of RGD.
Results:
After incubation with 100-300 mg/ml of RGD peptide for 24h, the VEGF content and the activity of PKC in experimental group was significantly higher than that in control group (P < 0.01).
Conclusion:
The stimulation of RGD peptide to the secretion of VEGF from VEC is in a dose-dependent way, and the stimulation is regulated by activating PKC.
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