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Related Experiment Video

Updated: Jun 14, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
07:37

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Decoding circulating nucleic acids in human serum using microfluidic single molecule spectroscopy.

Kelvin J Liu1, Malcolm V Brock, Ie-Ming Shih

  • 1Biomedical Engineering Department, 3400 North Charles Street, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Journal of the American Chemical Society
|April 7, 2010
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel, one-step assay for analyzing circulating nucleic acid (CNA) size and quantity in human serum. The microfluidic technique offers a rapid, inexpensive alternative to PCR for CNA analysis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Circulating nucleic acids (CNA) are valuable noninvasive biomarkers.
  • DNA fragment size is a promising marker for cancer and prenatal diagnostics.
  • Current CNA analysis methods often require complex procedures like PCR.

Purpose of the Study:

  • To develop a rapid, facile, and inexpensive one-step assay for analyzing circulating DNA size and quantity directly in human serum.
  • To demonstrate the utility of microfluidic single molecule spectroscopy for CNA analysis.

Main Methods:

  • Developed a one-step assay using microfluidic cylindrical illumination confocal spectroscopy.
  • Employed fluorescence burst size analysis to individually count and size fluorescently-labeled CNA molecules.

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Related Experiment Videos

Last Updated: Jun 14, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
07:37

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Published on: June 26, 2016

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

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06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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  • Established a size calibration curve using lambda Hind III digest DNA (564 bp to 27.5 kbp).

    • Main Results:

    • Achieved a linear relationship between DNA length and burst size from 564 bp to 27.5 kbp.
    • Optimized single molecule assay parameters for serum samples.
    • Successfully performed DNA sizing analysis directly in patient serum without DNA isolation or amplification.

    Conclusions:

    • Microfluidic single molecule spectroscopy provides a rapid, facile, and inexpensive alternative to PCR-based CNA analysis.
    • This assay enables direct analysis of circulating DNA size and quantity in human serum.
    • The developed method holds potential for improved cancer and prenatal diagnostics.