Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

The Tree of Life - Bacteria, Archaea, and Eukaryotes

The Tree of Life - Bacteria, Archaea, and Eukaryotes

The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both extant and...

Cooperative Allosteric Transitions

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Panorama: A robust pangenome-based method for predicting and comparing biological systems across species.

PLoS computational biology·2026

Same author

Author Correction: Magnetoreception in a freshwater ciliate arises from endosymbiosis.

Nature communications·2026

Same author

Magnetoreception in a freshwater ciliate arises from endosymbiosis.

Nature communications·2026

Same author

Identification of specific metabolic capacities associated with major extraintestinal pathogenic <i>Escherichia coli</i> lineages.

Journal of bacteriology·2026

Same author

Concomitant production of exopolysaccharides and carotenoids by the UVC-resistant bacterium <i>Kocuria rosea</i> CV6.

International journal of radiation biology·2026

Same author

Characterization of Frankia casuarinae Mutants Defective in Vesicle Envelope Development.

Microbes and environments·2025

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

The Frankia alni symbiotic transcriptome.

Nicole Alloisio¹, Clothilde Queiroux, Pascale Fournier

¹Université de Lyon, Lyon, France.

Molecular Plant-Microbe Interactions : MPMI

|April 7, 2010

Summary

Frankia bacteria form root nodules, converting nitrogen to ammonia for plants. Their symbiotic gene expression is conserved across plant families and more active than rhizobia.

More Related Videos

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Published on: May 9, 2017

Fecal (micro) RNA Isolation

Fecal (micro) RNA Isolation

Published on: October 28, 2020

Related Experiment Videos

Last Updated: Jun 14, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Published on: May 9, 2017

Fecal (micro) RNA Isolation

Fecal (micro) RNA Isolation

Published on: October 28, 2020

Area of Science:

Microbiology
Plant Science
Genomics

Background:

Actinobacteria Frankia spp. form symbiotic root nodules with actinorhizal plants, fixing atmospheric nitrogen into ammonia.
This symbiosis is crucial for plant nutrition and ecosystem nitrogen cycling.

Purpose of the Study:

To analyze the symbiotic transcriptome of Frankia alni in root nodules of Alnus glutinosa.
To compare gene expression patterns between free-living and symbiotic Frankia.
To investigate the conservation of symbiotic gene expression across different plant families.

Main Methods:

Whole-genome microarray analysis of nitrogen-replete free-living Frankia alni and Alnus glutinosa nodule bacteria.
Transcriptional profiling to identify differentially expressed genes during symbiosis.

Main Results:

Nodule-induced genes were located in syntenic genomic regions, while repressed genes were not conserved.
Key nitrogen fixation genes (nif, hup2, suf, shc) were highly induced.
Genes related to ammonium assimilation and transport showed modified expression, suggesting limitations.
Symbiotic transcriptome was conserved across Betulaceae and Myricaceae plant families.
Frankia alni exhibited higher metabolic activity in symbiosis compared to rhizobia.

Conclusions:

Frankia symbiosis involves coordinated regulation of specific gene sets, including nitrogen fixation and transport.
The symbiotic transcriptome is conserved across diverse plant hosts, indicating a robust symbiotic mechanism.
Frankia alni demonstrates a highly active metabolic state during symbiosis, potentially exceeding that of rhizobia.