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Updated: Jun 14, 2026
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
Published on: March 15, 2018
Inhibition of the osteoclast activity with the application of recombinant murine RANK protein
Peifu Tang1, Licheng Zhang, Meng Xu
1Chinese PLA General Hospital, Department of Orthopedics, Beijing, PR China.
Objective:
To investigate the effects of recombinant Murine RANK on the osteoclast activity.
Methods:
Osteoclast was observed with soluble RANK. Female Wistar rats were bilaterally ovariectomized, and intra-abdominally injected with 5 mg/Kg soluble RANK. The bone metabolism, bone density, and bone histomorphology were observed.
Results:
Compared with the control group, the quantity of TRAP-positive osteoclasts and bone resorption pit counting in the rest groups were significantly reduced. The bone density of the dosed groups was significantly increased and TRAP-stained osteoclasts in bone tissue sections were almost inhibited.
Conclusion:
rh-Murine RANK was able to inhibit osteoclast differentiation and prevent ovariectomy-induced bone loss.
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