Related Experiment Video
Updated: Jun 14, 2026
Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
Published on: May 17, 2019
[Relationship between proteoglycans and proliferation of human salivary adencid cystic carcinoma]
Hong Shi1, Jie Wang, Fu-Sheng Dong
1Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
Objective:
To investigate the effect of down-regulated proteoglycans on the proliferation of human salivary adenoid cystic carcinoma (SACC).
Methods:
The short hairpin RNA (shRNA) plasmid silencing human xylosyltransferase-I (XT-I) gene was constructed and named shRNA-WJ3. Adenoid cystic carcinoma cells with high metastatic tendency (ACC-M) were transfected by shRNA-WJ3. The plasmid shRNA-HK not targeting any human gene was transfected into ACC-M cells used as negative control. After 48 h of transfection, the positive cells were screened by G418 to isolate the stable transfected cells. Real-time PCR and Western blotting were used to test the gene silence, and the proteoglycans contents of the cells were detected. The stable cell line silenced XT-I was named ACC-M-WJ3. MTT assay was performed to detect the cell proliferation. The cell cycle was analyzed by flow cytometry.
Results:
ShRNA-WJ3 showed powerful RNA interference and gene silence of XT-I. The inhibition rate was 83.70% of mRNA expression and 79.60% of protein expression respectively. The content of proteoglycans in ACC-M-WJ3 was down-regulated by 49.71%-54.59%. The results of MTT assay showed that the cell growth was inhibited significantly. S phrase decreased and G₁-G₀ phrase increased in group ACC-M-WJ3 compared with that of group ACC-M-HK (P < 0.05).
Conclusions:
The down-regulated proteoglycans could inhibit the proliferation of human ACC-M cells.
Insights
Down-regulating proteoglycans by silencing the xylosyltransferase-I (XT-I) gene significantly inhibited human adenoid cystic carcinoma cell proliferation. This suggests proteoglycans are key drivers of SACC growth.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Context:
- Salivary adenoid cystic carcinoma (SACC) is an aggressive cancer.
- Proteoglycans play a role in cancer progression.
- The role of proteoglycans in SACC proliferation requires further investigation.
Purpose:
- To investigate the effect of down-regulated proteoglycans on human SACC proliferation.
- To determine if targeting proteoglycan synthesis can inhibit SACC growth.
Summary:
- Short hairpin RNA (shRNA) targeting the human xylosyltransferase-I (XT-I) gene was used to down-regulate proteoglycans in SACC cells (ACC-M).
- Gene silencing of XT-I resulted in significant reduction of proteoglycan content.
- MTT assays and flow cytometry demonstrated inhibited cell proliferation and cell cycle arrest in the treated SACC cells.
Impact:
- This study reveals that down-regulated proteoglycans inhibit SACC cell proliferation.
- Targeting proteoglycan synthesis presents a potential therapeutic strategy for SACC.
- Further research into proteoglycan-targeted therapies for SACC is warranted.
Related Concept Videos
01:05Proteoglycans
02:23Abnormal Proliferation