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Updated: Jun 14, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
[The diagnostic value of fluorescence quantitative PCR in screening of influenza A virus]
Yu-ling Shi1, Yong-quan Yang, Lan-lan Zeng
1Department of Clinical Laboratory, General Hospital of Guangzhou Command, Guangzhou 510010, China. shiyuling6@sina.com
Aim:
To discuss the the diagnostic value of fluorescence quantitative PCR in screening of influenza A virus.
Methods:
Influenza A virus RNA in 150 cases of suspected influenza patients with throat swab was measured by fluorescence quantitative RT-PCR. The white blood cell count was measured by XT-1800 automated hematology analyzer in patients' anticoagulant EDTA whole blood. 45 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold.
Results:
In 150 patients, 54 cases were detected influenza A virus RNA positive, the positive rate was 36%.The 54 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold, the results were all negative.The white blood cell count is (6.81+/-2.12) x 10(9)/L in the influenza A virus RNA-positive patients, and (6.64+/-3.13) x 10(9)/L for the negative cases. White blood cell count in patients with influenza A and non-influenza patients have no significant difference.
Conclusion:
Fluorescence quantitative RT-PCR in the detection of influenza A virus RNA has a better positive rate, its sensitivity and specificity are better than colloidal gold and white blood cell count, and it can be screened quickly and efficiently in patients with influenza to prevent disease outbreaks.
