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Detection of RTP801, a gene that is differentially expressed in irradiated HeLa cells
Young-Sook Lee1, Moon-June Cho, Jeung Hoon Lee
1Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
Purpose:
To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells.
Materials And Methods:
Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye.
Results:
RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation.
Conclusions:
These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells.
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