Related Experiment Video

Updated: Jun 14, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

DNA and general PCR methods: allele-specific PCR

Kristie Nybo

    Biotechniques
    |April 8, 2010
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes08:35

    Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

    Published on: July 17, 2021

    Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions08:23

    Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

    Published on: September 25, 2018

    Related Concept Videos

    DNA Isolation01:24

    DNA Isolation

    DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
    PCR - Polymerase Chain Reaction01:32

    PCR - Polymerase Chain Reaction

    Overview
    PCR01:32

    PCR

    Overview
    Sanger Sequencing01:57

    Sanger Sequencing

    DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
    Real Time RT-PCR02:57

    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
    The real-time quantification of the number of amplified products is...
    RACE - Rapid Amplification of cDNA Ends02:35

    RACE - Rapid Amplification of cDNA Ends

    Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
    Since the...