Related Experiment Videos
RANK/RANKL expression is induced by cardiac surgical operation
Jan Krejsek1, Martina Kolácková, Vladimír Lonský
1Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Czech Republic. krejsek@fnhk.cz
Insights
Cardiac surgery increases RANK and RANKL expression on monocytes. Mini-invasive surgery showed significantly higher expression of these immune regulators post-operation.
Area of Science:
- Immunology
- Cardiovascular Surgery
- Bone Metabolism
Background:
- Cardiac surgery triggers a systemic inflammatory response.
- RANK/RANKL molecules are key regulators of bone metabolism and immune function.
Purpose of the Study:
- To investigate changes in Receptor Activator of Nuclear factor Kappa-B (RANK) and its ligand (RANKL) expression on innate immune cells in cardiac surgical patients.
- To compare RANK/RANKL expression between conventional "on-pump" and "mini-invasive on-pump" coronary artery bypass grafting.
Main Methods:
- Flow cytometry was used to analyze RANK/RANKL expression.
- Twenty-six patients undergoing coronary artery bypass grafting were studied.
- Patients were divided into "on-pump" and "mini-invasive on-pump" groups.
Main Results:
- Monocyte RANK and RANKL expression significantly increased post-surgery in "mini-invasive on-pump" patients (days 1, 3, 7).
- "On-pump" patients showed increased RANKL expression on postoperative day 3.
- No significant differences in RANK/RANKL expression were observed between the two surgical groups.
Conclusions:
- RANK and RANKL expression are significantly upregulated on monocytes following "mini-invasive on-pump" cardiac surgery.
- These findings highlight the role of RANK/RANKL in the inflammatory response after cardiac surgery.
Background:
Cardiac surgery provokes a systemic inflammatory response in any patient. This complex body reaction involves also RANK/RANKL molecules which have been recently identified as principal regulators of bone metabolism.
Aims:
To follow the changes in the expression of RANK/RANKL molecules on innate immune cells of cardiac surgical patients.
Patients And Methods:
Twenty-six patients undergoing cardiac surgical were assigned to undergo coronary artery bypass grafting using either cardiopulmonary bypass ("on-pump") or modified "miniinvasive on-pump". The expression of RANK/RANKL was performed by flow cytometry.
Results:
Significantly increased expression of RANK on monocytes of "miniinvasive on-pump" patients was found at the 1st, the 3nd, and 7th postoperative days. The similar pattern was found also for monocyte RANKL expression. In addition, RANKL expression was significantly increased at the 3rd postoperative day in "on-pump" patient. No significant differences between "miniinvasive on-pump" and "on-pump" cardiac surgical patients were found.
Conclusion:
The expression of both RANK and RANKL molecules is significantly enhanced on monocytes of "miniinvasive on-pump" cardiac surgical patients.
Related Concept Videos
01:28Cardiomyopathy VII: Pre and Post Operative Nursing Management
01:23Treatment for Pulmonary Arterial Hypertension: Prostacyclin Receptor Agonists
These agonists bind to the IPR receptor situated on the plasma membrane of the pulmonary artery smooth muscle cells. This binding triggers a cascade of reactions known as the GS-AC-cAMP-PKA pathway. This pathway results in the relaxation of smooth muscle...